DR. AYLIN ALTAY KOCAK2026-06-082026-06-08https://dspace.academy.edu.ly/handle/123456789/2216the disk diffusion (D test) test. According to D test, resistance phenotypes were detected and resistant phenotypes were screened for resistance genes using polymerase chain reaction (PCR). PCR amplification was made using primers specific for ermA, ermB, ermC, and msrA genes. Among 50 MRSA isolates, 25 (50%) isolates were resistant to erythromycin with D zone positivity and susceptible to clindamycin indicating inducible iMLSB phenotype. And 2 (4%) isolates were resistant to clindamycin and erythromycin without the D zone indicating constitutive cMLSB phenotype. Twenty-two (44%) isolates were susceptible to clindamycin and erythromycin with D-zone negative indicating S phenotypeMethicillin-resistant Staphylococcus aureus strains pose a serious treatment problem because of their multidrug resistance. The rapid transmission of the erm gene leading to macrolide lincosamide streptomycin B (MLSB) resistance severely limits the clinical application of traditional macrolides such as erythromycin. In this study, we aimed to characterize the MLSB phenotype and genotypic resistance genes of MRSA and MSSA isolates from Baskent University Hospital, and resistance rates of MSSA and MRSA isolates were compared. The study included 50 MSSA and 50 MRSA isolates collected between 2016 and 2022 from Baskent University in Ankara and Adana Hospitals. First, the S. aureus isolates were confirmed by catalase and coagulase tests, and the MRSA and MSSA isolates were confirmed by cefoxitin disc diffusion testSTREPTOGRAMIN B RESISTANCE AMONG METHICILIN RESISTANT