UNIVERSITY OF CYBERJAYA2024-12-162024-12-16https://dspace.academy.edu.ly/handle/123456789/887The antibacterial activity using the agar well diffusion method showed that the novel AU-23 compound possesses remarkable antibacterial activity against four reference ATCC strains (Pseudomonas aeruginosa ATCC 9027, methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591, methicillin-sensitive Staphylococcus aureus(MSSA) ATCC 25923, and methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300) that commonly cause wound infection, with an average inhibition zone ranging between 12mm and 18mm. The MIC and MBC of the AU-23 compound against the ATCC strains ranged from 15.63 to 62.5 µg/mL. Moreover, the dose-dependent AU- 23 exhibited bactericidal activity against two sensitive ATCC bacterial strains, P. aeruginosa 9027 and MSSA 25923, during the first 4 and 6 hours. Antibiofilm activity using the XTT assay was significantly higher against both ATCC strains, MSSA 25923 and P. aeruginosa 9027, when grown in mono-microbial biofilm mode, with inhibition rates of 64.98% and 59.16%, respectively. However, prenylated flavonoid PF-23 showed a slightly good antibacterial effect against MSSA25923 and P. aeruginosa 9027, with inhibition zones ranging from 10 to 11mm, and MIC/MBC values between 500 and 1000 µg/mL.ABSTRACT Wounds affect around 120 million of the global population, with 18 million remaining unresolved a year post-injury. This widespread presence of chronic wounds, coupled with the worldwide increase in surgical procedures, is fuelling the need for wound management and healing products. It was found that about 8.2 million Medicare beneficiaries suffered from at least one type of wound infection. Several medications have been widely used to treat wounds, but some of these agents are associated with undesirable outcomes and have caused serious public health concerns. Generally, aurones and prenylated flavonoids have been used as therapeutic agents exhibiting antioxidant, anticancer, anti-inflammatory, antimicrobial, and antidiabetic activity. This study evaluated the antibacterial, anti-inflammatory, and wound-healing properties of novel synthetic aurone (AU-23) and prenylated flavonoid (PF-23) by in vitro and in vivo studies.EVALUATION OF ANTIBACTERIAL, ANTIINFLAMMATORY AND WOUND HEALING PROPERTIES OF NOVEL SYNTHETIC AURONE (AU-23) AND PRENYLATED FLAVONOID (PF-23): IN VITRO AND IN VIVO STUDIES