Dr .Tim Robbins2025-10-282025-10-28https://dspace.academy.edu.ly/handle/123456789/1816The relative influence of seed set production was also investigated. Two petunia genotypes were used as twelve plants grown from cuttings in total. They were grown in the glasshouse under room conditions at 22°C for a period of 16/8 hr in a supplementary lights cycle, and then three plants of p, hybrida and three of p, inflata were transferred to a different glasshouse room where they were exposed to high temperature ranging from 28°C to 34 °C in order to acclimatise them before examining the high temperatures possible effects on both pollen tube growth and seed set production. Pollen tubes growth was positively influenced by high temperature during pollination, howeverno seed set produced. Reverse transcriptional PCR results show that the S-RNases expression level is also affected by high temperature although small sample size was used. Although a little evidence of the breakdown of GSI is provided by these findings, the breakdown of GSI in both P. hybrida and P. inflata is achievable by high temperature, in particular if larger project scale can be applied under more precise temperature conditions in special growth rooms. These findings are also consistent with what is achieved in the past studies with other plant species that are of GSI system control.GSI in Petunia is a genetically controlled mechanism for rejection of own pollen. It has been a favourite topic for botanists and geneticists since Darwin. Within the last three decades, molecular and biochemical analyses have significantly contributed to the elucidation of the complex series of interactions occurring at the pollen–stigma interface. Molecular analyses of GSI system have focused on identifying and characterising the pollen and pistil components of the self-incompatible response as well as other proteins and events that lead to pollen rejection.Breakdown of Self-incompatibility