مشروع البحث: Survey on urban Mosquito( Diptera:Culicidace of souq AL juma Municipality in Tripoli Libya
تحميل...
المساهمين
الممولين
رقم التعريف
6954
الباحث
حنان العربي عقيلة
الوصف
Mosquitoes of the family Culicidae are distributed worldwide with more than 3557 species. A Mosquito survey (Diptera: Culicidae) was conducted at Souq Al-Jum‟aa Municipality, Eastern region of Tripoli. During the period from June to December 2016, the survey aimed to identify the most common mosquito‟s species and the composition of mosquitoes in the study area. Culex pipiens is the most widespread mosquito vector in temperate regions including North Africa. It was the most common in this study. Culex pipiens have two recognized biotypes and hybrids; pipiens and molestus these sibling species transmit pathogens by exhibiting intermediate physiological and behavioral traits, and are considered of great epidemiological importance. The coexistence of species complexes (sibling and cryptic species) could create problems in mosquito control because different members of a complex are likely to differ in their involvement in disease transmission and their response to various control methods. For this reason, in the study, we prospected for the presents of Culex pipiens biotypes (Cx. pipiens pipiens & Cx. pipiens molestus) and their hybrid. The mosquito field survey was conducted by using CDC - Ultra Violet light trap from 12 sites at Souq Al -jum‟aa municipality - Tripoli / Libya, during the summer and autumn period of 2016. Mosquitoes were morphologically identified by Stereomicroscope with objectives W- PI 10x, 23 and using offline MosKeyTool: an interactive identification key for mosquitoes of Euro-Mediterranean. Molecular identification is used to distinguish between Culex pipiens forms by using diagnostic primers designed for the flanking region of microsatellite CQ11, and Simple Chelex Protocol for DNA Extraction were used according to microscopic examination of the collected mosquito samples, Shannon diversity index was used to give a complete picture of diversity and the abundance of species and richness of the mosquito sample in the study area. In all, seven species were collected that belong to 3 genera namely; Culex 96% (3 species); Aedes 3.3% (3 species) and Culiseta 1.5% (one species), the study revealed that Maximum species richness was observed as well as mosquito population abundance was recorded in Arada Rd 1, 16th Road, Alhany Rd and Talpagh respectively. This study included 7 species; six species have medical importance (Aedes detritus, Aedes caspius, Aedes dorsalis, Culex perexiguus, Culex pipiens and Culiseta longiareolate) were as Culex laticinctus has no medical importants. The most abundant mosquito genus in the twelve study sites was Culex, Culex pipiens with 84% which was the most abundant species. We established the presence of both forms of Culex pipiens and their hybrids in municipality by using Simple Chelex Protocol for DNA Extraction. Our results show that Simple Chelex Protocol method can be substituted as a simple and sustainable approach in
XI
resource-limited settings where a constant reagent supply chain is often difficult to maintain. The Agarose gel Electrophoresis results of PCR assay revealed 51% of adults tested were pure for the 200 bp fragment which is characteristic of the pipiens forms 35% were pure for the 250 bp fragment identifying the molestus form and the remaining 14 % corresponded to hybrids (200/250bp).
الكلمات الدالة
Survey on urban Mosquito( Diptera