مشروع البحث: Biochemical and Histopathological effects of iron, folate overload on liver and spleen in offspring mice
تحميل...
المساهمين
الممولين
رقم التعريف
24213
الباحث
ملاك عمران علي دخيل
الوصف
Introduction: Anemia during pregnancy is an important risk factor for maternal, fetal and neonatal mortality, poor pregnancy outcomes, and delayed cognitive development, especially in underdeveloped countries. Most pregnant women find it difficult to consume sufficient iron (Fe) in their diets to meet their basic needs. This has led to a global policy of Fe supplementation without prior screening of Fe and ferritin in the body. The objective of this study is to investigate the effect of Fe and folic acid FA overload on the hematopoietic function, liver function and kidney function of mice and their offspring, as well as its effect on the fertility of female mice and their reproductive hormones. It also investigated the effect on the mortality rate of mice offspring. The animals were raised and provided with appropriate conditions for reproduction in an animal house at the Faculty of Veterinary Medicine, University of Tripoli, until the required number for the experiment was obtained. The doses were given to 36 female mice only, which were divided into 6 groups: the control group and 5 other groups were divided as follows: Group I (control), Group II (Fe 15mg), Group III (FA 4mg), and Group IV (FA 8mg). Group V (Fe with FA 4mg) and Group VI (Fe with FA 8mg). Daily intraperitoneal injections of Fe were administered, and oral FA supplements were provided for a week following the mice's mating. Mice were monitored, weighed weekly, and fed daily until birth. Then care for the newborns until the age of weaning (one month after the age of weaning). The results showed that female mice that were exposed to high doses of Fe, alone or with FA, became infertile, but when Fe was given with FA, FA had a clear protective effect on liver enzymes. Fe overload also causes side effects on the functions of the liver, kidney and weight of the liver and spleen. While FA had a clear effect on increasing the fertility of mice, high doses of FA caused side effects on liver and spleen functions and increased the mortality rate of the offspring. Conclusion
الكلمات الدالة
Biochemical and Histopathological effects of iron, folate overload on liver and spleen in offspring mice