مشروع البحث: Diagnostic Evaluation between ELLSA and Rapid Screening Technoques for anti_HCV among Blood Donor in libya
| dc.contributor.advisor | د. عبد الرحمن محمد فرارة | |
| dc.date.accessioned | 2025-03-19T11:16:43Z | |
| dc.date.available | 2025-03-19T11:16:43Z | |
| dc.description | This study was conducted to determine the accuracy of the rapid technique compare with ELISA technique from (November, 2017 to June, 2018). A total of 400 samples were collected from blood donors at the blood banks center in the selected area (Tripoli, Benghazi and Sabha), were screened by using ELISA technique. The first 200 samples were retested by using Rapid test 1 for HCV 35 (17.5%) were positive for anti-HCV using ELISA, 15(7.5%) were positive using rapid test 1. Of the 35 ELISA positive samples, rapid test 1 detected 13 positives, giving 22 false negative, while, of the 165(82.5%) ELISA negative samples screened, 163 were negative and 2 were false positve. The second 200 samples were retested by using Rapid test 2 approximately 22 (11%) were positive for anti-HCV using ELISA, 18 (9%) were positive using rapid test 2. Of the 22 ELISA positive samples, rapid test 2 detected 4 positives, giving 18 false negative, while of the 178 (89%)ELISA negative samples screened, 164 were negative and 14 were false positive. Rapid test 1 showed 37.1% sensitivity and 98.8% specificity. Rapid test 2 showed 18.2% sensitivity and 92.1% specificity. Our Results showed remarkable sensitivity difference between rapid test and ELISA and the latter is found to be more accurate and sensitive. The study concluded that the sensitivity of rapid test used in this study was unsuitable and was inferior in quality testing of infectious markers in blood donors in blood bank as screening methods. So the HCV detection through rapid test should not be used as sole criteria for diagnosis of Hepatitis C especially in blood donor. | |
| dc.description.abstract | This study was conducted to determine the accuracy of the rapid technique compare with ELISA technique from (November, 2017 to June, 2018). A total of 400 samples were collected from blood donors at the blood banks center in the selected area (Tripoli, Benghazi and Sabha), were screened by using ELISA technique. The first 200 samples were retested by using Rapid test 1 for HCV 35 (17.5%) were positive for anti-HCV using ELISA, 15(7.5%) were positive using rapid test 1. Of the 35 ELISA positive samples, rapid test 1 detected 13 positives, giving 22 false negative, while, of the 165(82.5%) ELISA negative samples screened, 163 were negative and 2 were false positve. The second 200 samples were retested by using Rapid test 2 approximately 22 (11%) were positive for anti-HCV using ELISA, 18 (9%) were positive using rapid test 2. Of the 22 ELISA positive samples, rapid test 2 detected 4 positives, giving 18 false negative, while of the 178 (89%)ELISA negative samples screened, 164 were negative and 14 were false positive. Rapid test 1 showed 37.1% sensitivity and 98.8% specificity. Rapid test 2 showed 18.2% sensitivity and 92.1% specificity. Our Results showed remarkable sensitivity difference between rapid test and ELISA and the latter is found to be more accurate and sensitive. The study concluded that the sensitivity of rapid test used in this study was unsuitable and was inferior in quality testing of infectious markers in blood donors in blood bank as screening methods. So the HCV detection through rapid test should not be used as sole criteria for diagnosis of Hepatitis C especially in blood donor. | |
| dc.identifier | 7118 | |
| dc.identifier.uri | https://dspace.academy.edu.ly/handle/123456789/1564 | |
| dc.subject | Diagnostic Evaluation between ELLSA and Rapid Screening Technoques for anti_HCV among Blood Donor in libya | |
| dc.title | Diagnostic Evaluation between ELLSA and Rapid Screening Technoques for anti_HCV among Blood Donor in libya | |
| dspace.entity.type | Project | |
| project.endDate | 2019 | |
| project.funder.name | الأحياء الدقيقة | |
| project.investigator | نهي نور الدين حميس بوكاري | |
| project.startDate | 2018 | |
| relation.isOrgUnitOfProject | 2a4bb5ff-48b1-4f80-b63a-91de3ccabeb4 | |
| relation.isOrgUnitOfProject.latestForDiscovery | 2a4bb5ff-48b1-4f80-b63a-91de3ccabeb4 |