مشروع البحث: Association between Interleukin IL17A and IL17f gene polymorphisms and periodontal pathogens in chronic peridontitis among Libyans
| dc.contributor.advisor | نبيل صبري محمد النطاح | |
| dc.date.accessioned | 2025-03-27T10:27:40Z | |
| dc.date.available | 2025-03-27T10:27:40Z | |
| dc.description | Background: Chronic periodontitis (CP) is triggered by periodontal pathogens and influenced by genetic and environmental factors. Recent studies suggest that anti-inflammatory cytokines such as interleukin 17 (IL-17) plays a prominent role in the pathogenesis of CP. This study aims to investigate the association between CP and interleukin IL- 17 (A and F) gene polymorphisms. We also aim to evaluate the association between eight sub-gingival pathogens and CP among Libyans. Materials and Methods: The study consisted of 100 Libyan individuals between the ages of 25 and 65 years including 50 cases and 50 controls. DNA was extracted from buccal swabs and paper points for sub-gingival pathogen samples. IL 17 (A&F) genotyping was performed by PCR followed by Sanger sequencing. Specific 16S rRNA primers for each pathogen were applied in a multiplex PCR reaction and visualized by agarose gel electrophoresis for detection of sub-gingival pathogens. Results: A novel variant c.*34G>A in IL17F was found in 14.6% of patients and it was associated with CP (p-value = 0.010). IL17A GG and AG (rs2275913) genotypes were detected in patients with CP. IL-17F CT (rs763780) showed no association with CP (p-value = 0.334). Significant association between the eight sub-gingival pathogens and CP (p-value =0.0001) and high prevalence of sub-gingival pathogens in CP group. Conclusion: There is a strong association between eight sub-gingival pathogens and CP. More extensive genetic studies with a larger sample size are needed to further the association between interleukin-17 A and CP and the relation between novel variant c.*34G>A and CP among Libyans | |
| dc.description.abstract | Background: Chronic periodontitis (CP) is triggered by periodontal pathogens and influenced by genetic and environmental factors. Recent studies suggest that anti-inflammatory cytokines such as interleukin 17 (IL-17) plays a prominent role in the pathogenesis of CP. This study aims to investigate the association between CP and interleukin IL- 17 (A and F) gene polymorphisms. We also aim to evaluate the association between eight sub-gingival pathogens and CP among Libyans. Materials and Methods: The study consisted of 100 Libyan individuals between the ages of 25 and 65 years including 50 cases and 50 controls. DNA was extracted from buccal swabs and paper points for sub-gingival pathogen samples. IL 17 (A&F) genotyping was performed by PCR followed by Sanger sequencing. Specific 16S rRNA primers for each pathogen were applied in a multiplex PCR reaction and visualized by agarose gel electrophoresis for detection of sub-gingival pathogens. Results: A novel variant c.*34G>A in IL17F was found in 14.6% of patients and it was associated with CP (p-value = 0.010). IL17A GG and AG (rs2275913) genotypes were detected in patients with CP. IL-17F CT (rs763780) showed no association with CP (p-value = 0.334). Significant association between the eight sub-gingival pathogens and CP (p-value =0.0001) and high prevalence of sub-gingival pathogens in CP group. Conclusion: There is a strong association between eight sub-gingival pathogens and CP. More extensive genetic studies with a larger sample size are needed to further the association between interleukin-17 A and CP and the relation between novel variant c.*34G>A and CP among Libyans | |
| dc.identifier | 7187 | |
| dc.identifier.uri | https://dspace.academy.edu.ly/handle/123456789/1633 | |
| dc.subject | Association between Interleukin IL17A and IL17f gene polymorphisms and periodontal pathogens in chronic peridontitis among Libyans | |
| dc.title | Association between Interleukin IL17A and IL17f gene polymorphisms and periodontal pathogens in chronic peridontitis among Libyans | |
| dspace.entity.type | Project | |
| project.endDate | 2020 | |
| project.funder.name | الأحياء الدقيقة | |
| project.investigator | إشراق الشريف عبد الله الشريف | |
| project.startDate | 2019 | |
| relation.isOrgUnitOfProject | 2a4bb5ff-48b1-4f80-b63a-91de3ccabeb4 | |
| relation.isOrgUnitOfProject.latestForDiscovery | 2a4bb5ff-48b1-4f80-b63a-91de3ccabeb4 |