مشروع البحث: Comparative Analysis of Oral E.coli between Diabetic and Non-diabetic Dental Patients in Gharyan City
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المساهمين
الممولين
رقم التعريف
13-1
الباحث
أيوب محمد علي العربي
الوصف
The oral cavity of individuals with diabetes mellitus (DM) is a unique microenvironment susceptible to dysbiosis. While the link between diabetes and classic periodontal pathogens is well-established, the role of atypical opportunistic pathogens like Escherichia coli remains poorly understood, particularly in under-researched North African populations. This study aimed to conduct a comparative analysis of the prevalence and antimicrobial resistance of oral Escherichia coli in diabetic versus non-diabetic dental patients in Gharyan, Libya. A comparative cross-sectional study was conducted on 200 participants (100 with Type 2 DM and 100 non-diabetic controls). Data on demographics and clinical status were collected via a structured questionnaire. Oral samples were microbiologically analyzed using selective culture, with definitive bacterial identification and antimicrobial susceptibility testing performed using the BD Phoenix™ M50 automated system. Statistical analysis included Chi-square tests, Fisher’s exact test, and binary logistic regression. A significant disparity in microbial colonization was observed. Escherichia coli was found exclusively in the diabetic group, with a prevalence of 22.0% (p < 0.001). Conversely, Staphylococcus aureus was significantly more prevalent in non-diabetic controls (55.0% vs. 11.0%, p < 0.001). Within the diabetic cohort, a disease duration of over 10 years (OR = 2.86, 95% CI [1.10 – 7.54]) and a poor Plaque Index score (OR = 5.93, 95% CI [1.06 – 33.10]) were significant independent predictors for E. coli colonization. All 22 E. coli isolates were resistant to ampicillin, with 64.3% resistant to cefazolin, but remained susceptible to most modern broad-spectrum antibiotics. The diabetic oral cavity is a selective niche that promotes the colonization of opportunistic, drug-resistant enteric pathogens like E. coli. The risk is significantly amplified by the duration of the disease and poor oral hygiene. This positions the oral cavity as a clinically important reservoir for atypical pathogens in diabetic patients, underscoring the necessity of integrating rigorous oral healthcare into standard diabetes management. Keywords: Escherichia coli, Diabetes Mellitus, Oral Microbiome, Antimicrobial Resistance, Dysbiosis, Libya.
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