مشروع البحث: Investigating the Regulation of alternative splicing by extracellular matrix stiffness and cell density
تحميل...
المساهمين
الممولين
رقم التعريف
732
الباحث
أحمد مختار علي سالم
الوصف
ECM stiffness was found to modulate AS patterns of key cytoskeletal and signalling genes,
while high cell density reduced PTBP1’s nuclear localization, altering its splicing activity. A notable
discovery is the regulation of PTBP1 subcellular localization by cell density, which influences the
alternative splicing of NUMB. Specifically, we show that the inclusion of exon 9 (+E9) in NUMB
generates an isoform that promotes proliferation, enabling cells to override contact inhibition of
proliferation (CIP) to a certain extent. Conversely, the exclusion of exon 9 (ΔE9) produces an isoform
that enforces CIP,
الكلمات الدالة
cell density